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bioMerieux gmbh api 20ne test set
Api 20ne Test Set, supplied by bioMerieux gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/api+20ne+tests/api+20e/pmc11356111-103-12-17
Average 90 stars, based on 1 article reviews
api 20ne test set - by Bioz Stars, 2026-10
90/100 stars

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Article Title: A cold-adapted esterase from psychrotrophic Pseudoalteromas sp. strain 643A.
Article Snippet: A psychrotrophic bacterium producing a coldadapted esterase upon growth at low temperatures was isolated from the alimentary tract of Antarctic krill Euphasia superba Dana, and classiWed as Pseudoalteromonas sp. strain 643A.. A genomic DNA library of strain 643A was introduced into Escherichia coli TOP10F’, and screening on tributyrin-containing agar plates led to the isolation of esterase gene.. The esterase gene (estA, 621 bp) encoded a protein (EstA) of 207 amino acid residues with molecular mass of 23,036 Da.

Article Title: Antarctic marine bacterium Pseudoalteromonas sp. 22b as a source of cold-adapted beta-galactosidase.
Article Snippet: The marine, psychrotolerant, rod-shaped and Gram-negative bacterium 22b (the best of 41 b-galactosidase producers out of 107 Antarctic strains subjected to screening), classified as Pseudoalteromonas sp. based on 16S rRNA gene sequence, isolated from the alimentary tract of Antarctic krill Thyssanoessa macrura , synthesizes an intracellular cold-adapted b-galactosidase, which efficiently hydrolyzes lactose at 0 /20 8C, as indicated by its specific activity of 21 /67 U mg 1 of protein (11 /35% of maximum activity) in this temperature range, as well as kcat of 157 s , and kcat/Km of 47.5 mM 1 s 1 at 20 8C.. The maximum enzyme synthesis (lactose as a sufficient inducer) was observed at 6 8C, thus below the optimum growth temperature of the bacterium (15 8C).. The enzyme extracted from cells was purified to homogeneity (25% recovery) by using the fast, three-step procedure, including affinity chromatography on PABTG-Sepharose.

Article Title: Characterization of a Deep-Sea Actinobacterium Strain Uncovers Its Prominent Capability of Utilizing Taurine and Polyvinyl Alcohol
Article Snippet: Some of physiological characteristics were determined by using the API 20NE (Biomerieux, Lyon, France) tests.

Chromatography:

Article Title: Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens
Article Snippet: .. Gas chromatography-fatty acid methyl ester (GC-FAME) analysis and API 20NE tests (BioMerieux, S.A., Lyon, France) were performed to classify P. fluorescens strain SS101, one of the isolated Pseudomonas strains that caused lysis of zoospores. ..

Article Title: Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens
Article Snippet: After the normal distribution and homogeneity of variances were certified, root weight data and ranked disease index data were analyzed by analysis of variance, followed by Tukey's studentized range test (SAS Institute, Inc., Cary, N.C.). .. Gas chromatography-fatty acid methyl ester (GC-FAME) analysis and API 20NE tests (BioMerieux, S.A., Lyon, France) were performed to classify P. fluorescens strain SS101, one of the isolated Pseudomonas strains that caused lysis of zoospores. ..

Isolation:

Article Title: Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens
Article Snippet: .. Gas chromatography-fatty acid methyl ester (GC-FAME) analysis and API 20NE tests (BioMerieux, S.A., Lyon, France) were performed to classify P. fluorescens strain SS101, one of the isolated Pseudomonas strains that caused lysis of zoospores. ..

Article Title: Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens
Article Snippet: After the normal distribution and homogeneity of variances were certified, root weight data and ranked disease index data were analyzed by analysis of variance, followed by Tukey's studentized range test (SAS Institute, Inc., Cary, N.C.). .. Gas chromatography-fatty acid methyl ester (GC-FAME) analysis and API 20NE tests (BioMerieux, S.A., Lyon, France) were performed to classify P. fluorescens strain SS101, one of the isolated Pseudomonas strains that caused lysis of zoospores. ..

Lysis:

Article Title: Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens
Article Snippet: .. Gas chromatography-fatty acid methyl ester (GC-FAME) analysis and API 20NE tests (BioMerieux, S.A., Lyon, France) were performed to classify P. fluorescens strain SS101, one of the isolated Pseudomonas strains that caused lysis of zoospores. ..

Article Title: Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens
Article Snippet: After the normal distribution and homogeneity of variances were certified, root weight data and ranked disease index data were analyzed by analysis of variance, followed by Tukey's studentized range test (SAS Institute, Inc., Cary, N.C.). .. Gas chromatography-fatty acid methyl ester (GC-FAME) analysis and API 20NE tests (BioMerieux, S.A., Lyon, France) were performed to classify P. fluorescens strain SS101, one of the isolated Pseudomonas strains that caused lysis of zoospores. ..

Staining:

Article Title: Occurrence and characterization of psychrotolerant hydrocarbon-oxidizing bacteria from surface seawater along the Victoria Land coast (Antarctica)
Article Snippet: A total of 253 hydrocarbon-oxidizing bacterial isolates were achieved from eight Antarctic surface seawater samples enriched on diesel oil at 4°C.. Isolates were screened by ampliWed ribosomal DNA restriction analysis prior to 16S rRNA gene sequencing.. Sequences were compared to those in available databases using the Basic Local Alignment Search Tool network service to determine their approximate phylogenetic aYliations.

Starch:

Article Title: Occurrence and characterization of psychrotolerant hydrocarbon-oxidizing bacteria from surface seawater along the Victoria Land coast (Antarctica)
Article Snippet: A total of 253 hydrocarbon-oxidizing bacterial isolates were achieved from eight Antarctic surface seawater samples enriched on diesel oil at 4°C.. Isolates were screened by ampliWed ribosomal DNA restriction analysis prior to 16S rRNA gene sequencing.. Sequences were compared to those in available databases using the Basic Local Alignment Search Tool network service to determine their approximate phylogenetic aYliations.

Incubation:

Article Title: Isolation of Flavobacterium psychrophilum from sea lamprey, Petromyzon marinus L., with skin lesions in Lake Ontario.
Article Snippet: Diseases caused by Flavobacterium psychrophilum, a Gram-negative, long rod (0.2–0.75 · 1.5–7.5 lm) have recently become one of the most crucial problems affecting salmonid culture worldwide (Wood & Yasutake 1956; Pacha 1968; Lorenzen & Olesen 1997).. In North America, the disease is known as cold water disease (CWD) because of its occurrence usually at low water temperature among different species of salmonids.. The disease is characterized by skin erosions and ulcerations, darkening, fin and gill rot.



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Phenotypic characteristics of strain TAW-CT127.
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Emended description of Stutzerimonas chloritidismutans .

Journal: Microorganisms

Article Title: Genome-Based Taxonomy of the Genus Stutzerimonas and Proposal of S. frequens sp. nov. and S. degradans sp. nov. and Emended Descriptions of S. perfectomarina and S. chloritidismutans

doi: 10.3390/microorganisms10071363

Figure Lengend Snippet: Emended description of Stutzerimonas chloritidismutans .

Article Snippet: API 20NE differential tests for strain ATCC 17591 , Mannitol, maltose, gluconate..

Techniques: Isolation, Sampling, Incubation, Staining, Produced

Emended description of Stutzerimonas perfectomarina comb. nov.

Journal: Microorganisms

Article Title: Genome-Based Taxonomy of the Genus Stutzerimonas and Proposal of S. frequens sp. nov. and S. degradans sp. nov. and Emended Descriptions of S. perfectomarina and S. chloritidismutans

doi: 10.3390/microorganisms10071363

Figure Lengend Snippet: Emended description of Stutzerimonas perfectomarina comb. nov.

Article Snippet: API 20NE differential tests for strain ATCC 17591 , Mannitol, maltose, gluconate..

Techniques: Isolation, Sampling, Incubation, Staining, Produced

Phenotypic characteristics of strain TAW-CT127.

Journal: Microorganisms

Article Title: Aerobic Degradation Characteristics of Decabromodiphenyl ether through Rhodococcus ruber TAW-CT127 and Its Preliminary Genome Analysis

doi: 10.3390/microorganisms10071441

Figure Lengend Snippet: Phenotypic characteristics of strain TAW-CT127.

Article Snippet: Other than that, experiments to determine the utilization of different substrates by the strain were performed using the API 20NE bacterial metabolism test kit (BioMerieux).

Techniques: Staining, Starch

Differential characteristics of strain JM10B5a T and the closely related type strains of genus Pseudomonas .

Journal: Frontiers in Microbiology

Article Title: Pseudomonas oligotrophica sp. nov., a Novel Denitrifying Bacterium Possessing Nitrogen Removal Capability Under Low Carbon–Nitrogen Ratio Condition

doi: 10.3389/fmicb.2022.882890

Figure Lengend Snippet: Differential characteristics of strain JM10B5a T and the closely related type strains of genus Pseudomonas .

Article Snippet: Besides, stain JM10B5a T could be distinguished from the reference type strains by positive for adipic acid, trisodium citrate, esterase (C4), and lipase (C14) in the API 20NE and ZYM tests; and positive for D-galacturonic acid, D-glucuronic acid, gentiobiose, L-arginine, L-aspartic acid, L-galactonic acid lactone, mucic acid, quinic acid, α-hydroxybutyric acid, and γ-aminobutyric acid in the Biolog GNE III MicroPlate system.

Techniques: Concentration Assay